Neurobasal®-A Medium is a basal medium formulated to meet the special cell culture requirements of post-natal and adult brain neuronal cells when used withGIBCO® B-27® Supplement. Neurobasal®-A Medium can be used to grow neuronal cells from hippocampus, cortex and other regions of the brain. Neurobasal®-A Medium allows for both long and short term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer.
Add Your Own Supplements
All Neurobasal® media contain no L-glutamine, L-glutamic acid, or aspartic acid. Neurobasal® media must be supplemented with either a serum-free supplement (such asB-27® SupplementorN-2 Supplement), or with serum and 0.5 mML-glutamineorGlutaMAX™-I Supplement. For initial plating, you should add 25 µM glutamic acid.
Quality Control Testing
All Neurobasal® media are tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination. The osmolality of Neurobasal®-A was demonstrated to be optimal for postnatal and adult CNS neurons.
Neurobasal®-A Medium contains Phenol Red.
Neurobasal®-A Medium is optimized for post-natal and adult brain neurons.